α btan20 rat monoclonal dshb btan  (Developmental Studies Hybridoma Bank)

Journal: Cell Death & Disease

Article Title: Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition

doi: 10.1038/s41419-019-1973-7

Figure Lengend Snippet: a , b Western blots for Notch1 intracellular domain (NICD) and Rab7 after 72 h of treatment with various concentrations of test compounds in Jurkat ( a ) and Molt-4 cells ( b ). Tubulin is shown as a loading control. Blots are representative of three independent experiments. c Membrane, cytosolic, and whole-cell fractions of Rab7 protein. Western blots are representative of three independent experiments. d Impact of DGBP on NICD expression in other hematopoietic cell lines. Western blots are representative of three independent experiments. e , f Notch1 mRNA expression relative to 18S rRNA. Cells were treated with DGBP alone or a combination with geranylgeranyl diphosphate (GGPP) for 72 h. In all graphs, the bars represent means and standard deviations of three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA with Tukey’s post hoc analysis. * p < 0.05 versus the corresponding control

Article Snippet: The antibody for the Notch1 internal domain (bTAN20) was purchased from the Developmental Studies Hybridoma Bank (deposited by Artavanis-Tsakonas ).

Techniques: Western Blot, Control, Membrane, Expressing

Journal: Cell Death & Disease

Article Title: Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition

doi: 10.1038/s41419-019-1973-7

Figure Lengend Snippet: a , b Annexin V staining of Molt-4 or Jurkat cells treated with DGBP and co-treated with or without ( a ) DAPT or ( b ) MK-0752 for 72 h. Graphs represent means and standard deviations of three independent experiments. Statistical significance was determined by two-way ANOVA with Tukey’s post hoc analysis. * p < 0.05 versus untreated controls. ‡ p < 0.05 versus DGBP-treated condition. c , d Western blot of full-length Notch1 and NICD in Molt-4 cells following 72 h of treatment with DGBP and co-treatment with or without either the GGDPS product GGPP ( c ) or the caspase inhibitor Z-VAD-FMK ( d ). Tubulin is shown as a loading control. e , f Western blots of Notch1 and NICD after 72 h of treatment with DGBP and co-treatment with or without DAPT in Molt-4 ( e ) and Jurkat ( f ) cells. All Western blots are representative of three independent experiments

Article Snippet: The antibody for the Notch1 internal domain (bTAN20) was purchased from the Developmental Studies Hybridoma Bank (deposited by Artavanis-Tsakonas ).

Techniques: Staining, Western Blot, Control

Journal: Cell Death & Disease

Article Title: Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition

doi: 10.1038/s41419-019-1973-7

Figure Lengend Snippet: a Notch1 mRNA expression relative to 18S rRNA. Jurkat cells were treated with inhibitor alone or combinations for 72 h. b Luciferase activity in Jurkat cells transfected with Notch1 3′UTR. Cells were treated with inhibitor alone or co-treated with DGBP for 72 h. In all graphs, the bars represent means and standard deviations of three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA with Tukey’s post hoc analysis. * p < 0.05 versus the corresponding control. ‡ p < 0.05 versus DGBP-treated condition

Article Snippet: The antibody for the Notch1 internal domain (bTAN20) was purchased from the Developmental Studies Hybridoma Bank (deposited by Artavanis-Tsakonas ).

Techniques: Expressing, Luciferase, Activity Assay, Transfection, Control